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To conduct the study , the researchers used a mouse infected with a bacteria called Citrobacter rodentium - the mouse equivalent of an E.
Within two days of the mouse being infected, the researchers saw an increase in the permeability of the mouse's intestinal barrier - leading to water entering the intestines, causing diarrhea. This occurred well before inflammation cellular damage of the intestines. The researchers discovered two new proteins involved in causing diarrhea - interleukin and claudin-2, which humans possess too.
Pictured is the inside of a rat's colon. In the absence of a protein called claudin-2 whose presence leads to diarrhea, clearance of a infection-causing bacteria called C. Contracting F-actin cells of colon are in green, and DNA in blue. They found that when the mouse was infected, immune cells travelled to the intestinal wall and produced interleukin Interleukin binds to cells on the intestinal wall, causing the release of another protein called claudin It's claudin-2 that causes the leak in cellular junction in the intestinal wall, allowing water to enter it and cause diarrhea.
The researchers tested three different kinds of mice - regular mice, genetically modified mice that produce large amount of claudin-2, and mice that didn't make any claudin The regular mice had diarrhea when they got sick, and the mice that made more claudin-2 always had diarrhea.
The mice that didn't make any claudin-2 had more sever injuries to their intestinal lining, and they still had diarrhea because it seemed as though their immune system attacked the cells help make some diarrhea. The Bristol stool char is a diagnostic medical tool for classifying different forms of feces into seven different categories.
It's used in both clinical and experimental fields. Overall, the diarrhea induced by these two signalling molecules helped promote pathogen clearance and limited disease severity.
While some researchers have proposed limiting claudin-2 to prevent diarrhea, Dr Turner and his colleagues say that this signalling pathway may be critical for combating certain infections, especially in the early stages of a disease. It's still not clear if this mechanism is important in fighting all infections and if it's also important in humans, but since humans also have these proteins, the mouse model could serve as a useful preliminary study to human studies. It's still not clear if diarrhea is important in fighting all infections and if it's also important in humans, but since humans also have the same signalling proteins that were found in mice, the mouse model could serve as a useful preliminary study to human studies.
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